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- W2350598978 abstract "To construct a recombinant attenuated Salmonella typhimurium strain which can express tetanus toxin fragment C,the gene fragment encoding tetanus toxin fragment C was cloned into the expression vector pYA3333 containing asd gene,and a new recombinant plasmid pYA3333-TetC was generated.The recombinant plasmid pYA3333-TetC was transformed into an attenuated Salmonella(typhimurium) strain X4550 by electroporation,yielding the live vaccine strain X4550(pYA3333-TetC),which was a balanced-lethal recombinant.Stability and growth curve of the recombinant strain was determined.Results showed that the recombinant X4550(pYA3333-TetC) maintained good stability after 100 passages in vitro and the growth status of X4550(pYA3333-TetC) was the same as that of X4550(pYA3333).The recombinant strain X4550(pYA3333-TetC) was safe and could provide significant protection against a lethal challenge with tetanus toxin in immunized mice." @default.
- W2350598978 created "2016-06-24" @default.
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- W2350598978 date "2005-01-01" @default.
- W2350598978 modified "2023-09-26" @default.
- W2350598978 title "Construction and identification of recombinant Salmonella typhimurium strain expressing tetanus toxin fragment C" @default.
- W2350598978 hasPublicationYear "2005" @default.
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