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- W2350646095 abstract "In order to explore the mechanism and rule of action of Bordeaux mixture to soil catalase activity, effects of Bordeaux mixture on characteristic parameters and conformation of soil catalase were investigated using simulating method and fluorescence spectrum method. Results showed that 1)Soil catalase activity was stimulated by the low Bordeaux mixture concentrations and inhibited by the high Bordeaux mixture concentrations. The order of inhibition effect on the soil catalase with different Bordeaux mixture proportioning was multi-timesdouble-timeshalf-timesequal-times. The treatment which had the smallest effect on the soil catalase was the Bordeaux mixture treatment of 200mg·kg-1 and equal-times. 2)Under the culture time of 24h, Km and Vmax of enzymatic reaction were decreased at first and then increased with the increase of Bordeaux mixture concentrations. Statistical analysis indicated that Km and Vmax had significantly positive correlations with Bordeaux mixture concentrations, which implied that the Km values could reflect the degree of Bordeaux mixture affecting on soil catalase and Bordeaux mixture behaves as a typical uncompetitive inhibitor to soil urease in this experiment. 3)The influence of Bordeaux mixture on in vitro catalase was studied by molecular fluorescence spectrum. The Bordeaux mixture had a strong ability to quench the endogence fluorescence of in vitro catalase. According to the fluorescence quenching results between Cu (Ⅱ)and in vitro catalase, the binding constant K was 69.1831mol·L-1 and the number of binding site n was 0.6878." @default.
- W2350646095 created "2016-06-24" @default.
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- W2350646095 date "2009-01-01" @default.
- W2350646095 modified "2023-09-25" @default.
- W2350646095 title "Effects of Bordeaux Mixture on Soil Catalase Activity and Conformation Characteristics" @default.
- W2350646095 hasPublicationYear "2009" @default.
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