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- W2350654049 abstract "Ten B cell antigen epitopes were predicted according to the sequence of H and F protein genes of peste des retits ruminants virus(PPRV) by the computer software,and then selected by indirect-ELISA.The ten epitopes and an universal helper T lymphocyte epitope were synthesized one by one,these epitopes are compared by Glycin to keep independent,the recombinant multi-epitope antigen gene was inserted into the prokaryotic expression vector pET-30a(+),the recombinant pET-30(a) was transformed into Escherichia coli BL21(DE3) for expression.The molecular weight of expressed recombinant protein was about 19 ku.The purified protein could reacted with positive sera against PPRV in indirect-ELISA.The results showed that the recombinant plasmid was successfully constructed and the highly expressed protein could interact with standard positive serm of PPRV specifically.It may be a potential candidate of epitope vaccine against peste des petits ruminants." @default.
- W2350654049 created "2016-06-24" @default.
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- W2350654049 date "2011-01-01" @default.
- W2350654049 modified "2023-09-23" @default.
- W2350654049 title "Expression of multi-epitopes of H and F protein of peste des petits ruminants virus and analysis of their reactogenicity" @default.
- W2350654049 hasPublicationYear "2011" @default.
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