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- W2350659366 abstract "Two fragments of duck enteritis virus gB gene encoding major protective antigen regions were chosen on the basis of DNAStar software and obtained by PCR amplification.Then they were cloned into pMD18-T vector.After confirmed by PCR and restriction endonuclease analysis,the recombinant vectors were constructed using prokaryotic expression vector pET-32a(+).The recombinant plasmids were transformed into competent E.coli BL21(DE3) for further protein expression and the result of SDS-PAGE analysis indicated that the recombinant proteins could be expressed in inclusion body form at a high level in the presence of IPTG.The western-blotting analysis and ELISA showed the good antigenicity of the recombinant proteins." @default.
- W2350659366 created "2016-06-24" @default.
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- W2350659366 date "2008-01-01" @default.
- W2350659366 modified "2023-09-23" @default.
- W2350659366 title "Prokaryotic expression of duck enteritis virus gB gene and antigenicity analysis of the expressed product" @default.
- W2350659366 hasPublicationYear "2008" @default.
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