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- W2350712191 abstract "[Objective]To construct the expression plasmid of HIV-l gag and IFNα-2b fusion gene,observe the effect of co-expression of it and vaccinia virus,and study its significance. [Methods]By using gene recombination technique,IFNα-2b gene fragment was inserted into nt 531 site of gag gene,and the recombinant virus was selected by liposome transfection and screening of hemagglutinin negative plaque.The expression product was examined by immunofluorescence,Western blot and Dot ELISA. [Results]The indirect immunofluorescence assay showed that there was green fluorescence in the surface of cell with recombinant plasmid.The results of Western blot and Dot ELISA showed that there was gag/IFNα-2b protein in lysates of cell with recombinant plasmid. [Conclusion]The recombinant eukaryotic cells expression plasmid is successfully constructed,and expression protein has good immunogenicity and immune reactivity." @default.
- W2350712191 created "2016-06-24" @default.
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- W2350712191 date "2011-01-01" @default.
- W2350712191 modified "2023-09-23" @default.
- W2350712191 title "Study on Immunogenicity of HIV-1 gag/ IFNα-2b Expression Product" @default.
- W2350712191 hasPublicationYear "2011" @default.
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