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- W2350713605 abstract "A simple and sensitive method for the determination of free fatty acids has been developed by HPLC with fluorescence detection and mass spectrometric identification using a new reagent synthesized in our laboratory named 2-(12-oxobenzo[b]acridin-5(12H)-yl)ethyl 4-toluenesulfonate(BAETS) as pre-column labelling reagent.On a reversed-phased Hypersil BDS C8 column,34 fatty acid derivatives were separated and analyzed quantificationally.Fluorescent detection excitation and emission wavelengths were set at λex 273 nm and λem 503 nm,respectively.Correlation coefficients for fatty acid derivatives were more than0.9995,and detection limits(at signal-to-noise of 3:1) were in the range of 11.70 to 68.25 fmol.Free fatty acids from pollen samples were identified by the on line MS equipped with an atmospheric pressure chemical ionization source.When applying to the determination of free fatty acids from real samples,this developed method exhibited excellent sensitivity and reproducibility." @default.
- W2350713605 created "2016-06-24" @default.
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- W2350713605 date "2011-01-01" @default.
- W2350713605 modified "2023-09-25" @default.
- W2350713605 title "Determination of Saturated and Unsaturated Fatty Acids in Pollen by HPLC-FLD-MS with Fluorescent pre-column Derivatization" @default.
- W2350713605 hasPublicationYear "2011" @default.
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