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- W2350836031 abstract "Objective To establish the real-time RT-PCR method for detection of Borna disease virus(BDV) RNA.Methods The specific primers and the fluorescence probe of BDV-p40 were designed and synthesized;PCR products of BDV-p40 mRNA were cloned and used as standard template to develop the standard curve for samples detection;meanwhile the specificity and sensitivity of the method were performed.Results The real-time RT-PCR method for detection of BDV RNA was established and it could detect the total RNA of BDV infection cells which contained more than one copy of BDV genome.Conclusion The real-time RT-PCR method can be used to detect BDV infection and quantitatively analyse." @default.
- W2350836031 created "2016-06-24" @default.
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- W2350836031 date "2007-01-01" @default.
- W2350836031 modified "2023-09-28" @default.
- W2350836031 title "Establishment of real-time RT-PCR for detection of Borna disease virus RNA" @default.
- W2350836031 hasPublicationYear "2007" @default.
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