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- W2350868159 abstract "Immobilized trypsin was prepared with the Chitosan as matrix and glutaraldehyde as cross-linking agent through absorbing and crosslinking.The result of experiment manifested:the quality of enzyme,the concentration of the glutaraldehyde, pH, temperature and so on conspicuously effect on the activity of immobilized trypsin on Chitosan Microspheres.The best condition of the immobilization of trypsin:The quality of chitosan is 0.125 g, 14 mg of enzyme,the concentration of the glutaraldehyde is 0.2 %, pH=7.5,the temperature of immobilized enzyme is 35 ℃, the crosslinking time is 2 hours, the absorption time is 5 hours,.The activity recovery of immobilized enzyme is 76.57 %." @default.
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- W2350868159 date "2009-01-01" @default.
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- W2350868159 title "Study of condition of immobilized trypsin on chitosan microspheres" @default.
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