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- W2350871275 abstract "In order to study the molecular biology of PPV,the complete genome of porcine parvovirus(PPV) was amplified using polymerase chain reaction(PCR) method and cloned.The DNA fragment was sequenced and compared with reference porcine parvovirus.In addition,the major eptiope domain of porcine parvovirus structural gene VP2 was amplified by polymerase chain reaction(PCR) and inserted into the expression plasmid,pET-32a,then the recombination was induced by IPTG in Escherichia coli BL21.The NS1 gene between PPV YL strain and other porcine parvovirus strains showed 97.8%-99.4% and 96.7%-99.5% identities at nucleotide and amino acid levels respectively.The VP2 genes showed 98.7%-99.7% and 97.4%-99.8% homologies at nucleotide and amino acid levels respectively.The genetic relationship of PPV YL strain with BQ strain was the closest.SDS-PAGE and Western blotting analysis revealed that the recombinant protein was 56 ku.Biological activity of the recombinant protein was detected by Western blotting.The results can be applied in the genetic variation and differential diagnosis of PPV." @default.
- W2350871275 created "2016-06-24" @default.
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- W2350871275 date "2012-01-01" @default.
- W2350871275 modified "2023-09-25" @default.
- W2350871275 title "Sequence Analysis of Porcine Parvovirus YL Strain and Prokaryotic Expression of VP2" @default.
- W2350871275 hasPublicationYear "2012" @default.
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