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- W2350874604 abstract "Objective:To establish the RPB5-mediating protein(RMP)-silenced stable cell lines and study the inhibitory effects of small interfering RNA(siRNA) targeting RMP gene on the proliferation and migration of human hepatoma SMMC-7721 cells.Methods:Three RMPi siRNAs were designed and synthesized in vitro and transfected into SMMC-7721 cells.The inhibitory effect of siRNA on RMP gene expression was measured by RT-PCR to select the best siRNA.The expression vector pGPU6-Neo-RMP-484 was transfected into SMMC-7721 cells by the lipofectamine and the cells stably expressing the siRNA were selected by G418.RT-PCR was used to detect the interference efficacy against RMP gene.Cell proliferation and adhesion were measured by MTT assay.Wound healing test was used to observe the migration ability of cells.Results:The SMMC-7721 cell lines with down-regulated RMP expression were established by using RNA interference technology.Compared with the negative control cells,expression of RMP mRNA was down-regulated by(83.67±2.56)%.The proliferation of stable-transfected cells was inhibited by(74.33±0.58)%.The adhesion capability of stable-transfected cells was enhanced but the migration capacity was decreased compared with the negative control cells.Conclusion:The pGPU6-Neo-RMP-484 cell lines with stable transfection of RMP siRNA recombinant vector are successfully screened,which can be used as a cellular model for studying the molecular mechanism of RMP.Down-regulation of RMP gene expression can effectively inhibit the proliferation,enhance the adhesion,and decrease the migration of SMMC-7721 cells." @default.
- W2350874604 created "2016-06-24" @default.
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- W2350874604 date "2010-01-01" @default.
- W2350874604 modified "2023-09-24" @default.
- W2350874604 title "Effects of silencing RPB5-mediating protein(RMP) gene on cell proliferation and migration of liver cancer SMMC-7721 cells" @default.
- W2350874604 hasPublicationYear "2010" @default.
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