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- W2350878818 abstract "IL 15 and IL 15 receptors(IL 15R) play a crucial role in the development and progression of adult T cell leukemia(ATL), multiple myeloma and inflammatory autoimmune diseases. Recombinant DNA techniques were used to construct two targeted molecules that were designed to eliminate IL 15R overexpressing cells. A gene for human tumor necrosis factor mutant(TNFαM) was genetically fused to the DNA encoding wild type IL 15 or an IL 15 antagonist(IL 15M) and further cloned into pET16b under the control of T7 promoter, giving rise to pET IL 15 TNFαM and pET IL 15M TNFαM, respectively. Using Ni 2+ NTA affinity chromatography, IL 15/TNFαM and IL 15M/TNFαM were purified from E.coli BL21(DE3)plysS transformed with either pET IL 15 TNFαM or pET IL 15M TNFαM. The cytotoxicity of IL 15/TNFαM and IL 15M/TNFαM against IL 15R positive myelogenous leukemia cell line K562 was approximately 4 and 15 fold higher than that of recombinant TNFα, respectively. However, the cytotoxicity of both fusion constructs on IL 15R negative cell line Jurkat did not show marked difference and was relatively lower, compared to TNFα. The present data suggest that both protein fusions, especially IL 15M/TNFαM which is unable to trigger the activation of target cells, may have therapeutic potential in the treatment of diseases associated with abnormal expression of IL-15/IL-15R." @default.
- W2350878818 created "2016-06-24" @default.
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- W2350878818 date "2004-01-01" @default.
- W2350878818 modified "2023-09-25" @default.
- W2350878818 title "Construction and Characterization of TNFα-based Fusion Proteins Targeting Cells Overexpressing Interleukin 15 Receptors" @default.
- W2350878818 hasPublicationYear "2004" @default.
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