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- W2350888911 abstract "To make a designed neurochip practical,the adhesion and neurite growth of patterned striatal neurons were investigated by a microfluidic technique on the surface of mixture of polyethyleneimine(PEI) and laminin(LN) in vitro.Four different substrates,including LN,poly-L-lysine(PLL),PEI and mixture of PEI with LN(PEI+LN),characterized with strong positive surface charges were micropatterned on the silicon slide using a microfluidic technique.The striatal neurons from the forebrain of postnatal SD rats were cultured in vitro on these materials,respectively.The survival,adhesion and neurite growth of the cultured neurons,as well as their flow patterns on different substrates were evaluated,especially by immunofluorescent staining.Different effects of the neural flow patterns on different designs of the pattern(different spaces between the flow lines) fabricated on PEI+LN material were also compared using same method.The quantity of neuronal growth on the patterns of PEI,PLL and PEI+LN was apparently larger than that on LN.Moreover,the coated pattern of neurons on the PEI+LN was more integrated than on the others,and its morphology was more completely when the cells grew on the pattern with flow line spaces ranged between 150 and 200 μm.The substrate of PEI and LN mixture is in favor of creation of neural flow patterns,by using the microfluidic technology,when a suitable design of the pattern is selected.The observation in present study may help creation of some practical neurochips in the future times." @default.
- W2350888911 created "2016-06-24" @default.
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- W2350888911 date "2011-01-01" @default.
- W2350888911 modified "2023-09-22" @default.
- W2350888911 title "A Morphological Study on Creation of Neural Flow Patterns with Cultured Striatal Neurons in Vitro Using A Microfluidic Technique" @default.
- W2350888911 hasPublicationYear "2011" @default.
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