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- W2350973753 abstract "【Objective】To construct the bait expression plasmid pGBKT7-Survivin of survivin gene,which lay the foundations for constructing yeast two-hybrid system so as to screen target proteins of survivin protein.【Meth-ods】Total mRNA was extracted from human K562 cells,and then survivin cDNA was obtained by reverse transcrip-tion reaction.The ORF region of survivin gene was amplified,and then was ligated with the bait expression vector pGBKT7,the bait expression plasmid pGBKT7-Survivin was constructed ultimately.Then the bait expression plas-mid pGBKT7-Survivin was transformed into AH109 yeast cells,and the expression of the bait protein was analyzed by western blot.Toxicity and self-activation of the bait protein were detected simultaneously.【Results】Survivin-ORF was amplified and cloned into pGBKT7 successfully.After being verified by sequencing,the bait expression plasmid pGBKT7-Survivin was transformed into AH109 yeast cells successfully and no toxicity and self-activation were found.The high expression of the bait protein was confirmed by western blot.【Conclusion】The bait expres-sion vector pGBKT7-Survivin was constructed successfully,which lay the foundations for constructing yeast two-hy-brid system so as to screen target proteins of survivin protein." @default.
- W2350973753 created "2016-06-24" @default.
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- W2350973753 date "2008-01-01" @default.
- W2350973753 modified "2023-09-23" @default.
- W2350973753 title "Construction and identification of bait expression vector of Survivin gene" @default.
- W2350973753 hasPublicationYear "2008" @default.
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