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- W2350990321 abstract "Objective:To establish a method of specific chromatogram analysis on Radix Flemingiae by HPLC.Method:The HPLC method was used,chromatography condition were Waters Symmetry C18(4.6 mm×250 mm,5 μm)column,acetonitrile(A) –water(B) system as mobile phase with gradient elution(0-5 min,0%→5% A;5-10 min,5%→10% A;10-15 min,10%→30% A;15-25 min,30%→40% A;25-45 min,40%→80% A;45-80 min,80% A).The flow rate:0-5 min,0.6 mL·min-1;5-10 min,0.8 mL·min-1;10-80 min,1.0 mL·min-1.Detective wavelength was at 254 nm,column temperature was at room temperature.Use similarity evaluation system for chromatographic fingerprint of TCM to evaluate.Results:Similarity of 13 batches sample were 0.506-0.926,the specific chromatogram of Radix Flemingiae was established and 12 common peaks were indicated.Conclusion:This method is accurate and simple.It can be used as a quality control method for Radix Flemingiae." @default.
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- W2350990321 date "2009-01-01" @default.
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- W2350990321 title "HPLC specific chromatogram analysis of Radix Flemingiae" @default.
- W2350990321 hasPublicationYear "2009" @default.
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