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- W2351005057 abstract "Objective To explore the methods of effective transfection of CTLA 4Ig gene into rat kidneys and the effects of gene transfection on the survival time of renal allografts. Methods BN (Brown Norway, RT.1n) rats were randomized into 4 groups according to the methods of kidney transfection by CTLA 4Ig gene recombinant adenovirus (AdvCTLA 4Ig, 5×10 9 pfu/ml): transfection at 4 ℃ in vitro (group A), transfection via renal artery injection of AdvCTLA 4Ig (group B), transfection via renal artery injection of AdvCTLA 4Ig after ligation of the liver vessel and the aorta and the vena cava below the renal vessel (group C) and the control group (D). The transfection efficiency of AdvCTLA 4Ig was determined by immunofluorescence method. The kidneys with the highest transfection efficiency were transplanted into Lewis rats (transfection group). BN→Lewis kidney transplantation with non transplanted kidneys served as the controls. The allograft survival time was observed. Results The results showed that no specific fluorescence was observed in kidneys in group A, group B, and the control group. However, evident specific fluorescence was observed in kidneys in group C. The transfection occurred in tubular cells, but no transfection was observed in glomerular structure. The allograft survival time in transfection group was significantly longer than that of the controls [(26.6±6.9) d vs (8.1±1.6) d, P 0.01]. Conclusion The rat kidney (mainly the tubular cells) could be transfected efficiently by high titer AdvCTLA 4Ig in vivo , and the survival time of renal allografts could be prolonged by local transfection of CTLA 4Ig gene." @default.
- W2351005057 created "2016-06-24" @default.
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- W2351005057 date "2004-01-01" @default.
- W2351005057 modified "2023-09-25" @default.
- W2351005057 title "Effects of adenovirus-mediated CTLA-4Ig gene transfection into rat kidney on the surival rate of kidney as donor for transplation" @default.
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