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- W2351018686 abstract "Isolation of intact and pure cellular total RNA is very important to molecular cloning. However RNases are rich and stable in the nature, so RNA is liable to degradation caused by contamination from exogenous RNases. Rapid extraction of RNA reduces the chances of contamination from RNases. Three methods for direct and rapid extraction of total RNA are introduced in this paper, namely, method Ⅰ, methodⅡ and method Ⅲ. Method Ⅲ is the best. The RNA extracted with method Ⅲ is not degraded and of the highest degree of purity; three bands (28s, 18s and 5s) are clearly seen when checked with ethidiam-agrose gel electrophoresis. Method Ⅱ is not as good, a DNA band is observed in electrophoresis; and the RNA is degraded. Method Ⅰ can also extract RNA with comparatively high quality, but its purity is lower than that of the RNA extracted with method Ⅲ." @default.
- W2351018686 created "2016-06-24" @default.
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- W2351018686 date "2003-01-01" @default.
- W2351018686 modified "2023-09-25" @default.
- W2351018686 title "Extraction of Total RNA of Tissue Cells of Chicken Spleen" @default.
- W2351018686 hasPublicationYear "2003" @default.
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