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- W2351031328 abstract "Objective The paper is to explore the molecular mechanism of He Ne laser inducing apoptosis of scar fibroblasts in vitro. Methods These fibroblasts devired from hypertrophic scar (HS) were cultured and were divided into three groups:transfection group (TG),control transfection group (CTG) and non transfection group (NTG).The fibroblasts in TG were cotransfected with GFP (green fluorescent protein) cDNA and pcDNA3 FADD DN. Scar fibroblasts in CTG were cotransfected with GFP cDNA and pcDNA3. The fibroblasts in NTG was't transfected. Scar fibroblasts of each group were irradiated with He Ne laser (100 mW/cm2 for 30 minutes) at 24 hours after transfection, once a day for 3 consecutive days. Apoptosis of cell was then examined by TUNEL technique. Results The percent of apoptosis in TG was 9.27%.The percent of apoptosis was 15.73% in CTG, and 12.87% in NTG.The percent of apoptosis in TG was less than in CTG (q=4.55,P 0.01) and NTG (q=4.14,P 0.05) respectively. Conclusion FADD DN gene transfection can supress He Ne laser inducing apoptosis of scar fibroblasts.FADD may be an essential component of the He Ne laser activited cell death pathway." @default.
- W2351031328 created "2016-06-24" @default.
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- W2351031328 date "2002-01-01" @default.
- W2351031328 modified "2023-09-23" @default.
- W2351031328 title "Effects of FADD-DN gene transfection on He-Ne laser-inducing apoptosis of scar fibroblasts in culture" @default.
- W2351031328 hasPublicationYear "2002" @default.
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