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- W2351032491 abstract "Objective To construction the survivin-siRNA expression vector and to research the function of survivin in laryngeal squamous cell carcinoma.Methods According to survivin cDNA sequence in genebank,the target sequence was designed and synthesized.After annealing,oligonucleotides were inserted into pGenesil-1 vector,the recombined plasmid was identified by restriction endonuclease and ENA sequencing.Results By restriction endonuclease,eukaryotic expression plasmid of survivin proved to be successfully constructed.Sequence analysis of inserted fragment revealed the same sequence as synthesized oligonucleotides.Conclusion The survivin-siRNA expression vector has been successfully constructed.It is foundation for studying the function of survivin by small interfere RNA technique." @default.
- W2351032491 created "2016-06-24" @default.
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- W2351032491 date "2008-01-01" @default.
- W2351032491 modified "2023-09-28" @default.
- W2351032491 title "Construction and identification of eukaryotic expression vector of target survivin" @default.
- W2351032491 hasPublicationYear "2008" @default.
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