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- W2351036694 abstract "Based on the genome sequence of Newcastle disease virus(NDV) published in GenBank,ten pairs of gene-specific primers were designed to amplify cDNA of SHJ00 strain from diseased Sanhuang chicken by RT-PCR technique.Sequence analysis showed that the entire genome of SHJ00 consisted of 15 192 nucleotides.SHJ00 and NDV strains shared 6 common ORFs with the same order:3′-NP-P-M-F-HN-L-5′,but SHJ00 genome had extra six nucleotides(ACACTC)between NP and P genes.The F gene was 1 672 bp and contained a complete open reading frame encoding a protein of 553 amino acids.The amino acid sequence of cleavage site region was 112R-R-Q-K-R-F117 which was the same as virulent NDV strains.The SHJ00 belonged to NDV genotype Ⅶ,and had 80.9%-97.7%nucleotide homology with other published NDV strains.The nucleotide sequence of HN gene was 1 716 bp and encoded a protein of 571 amino acids.It shared 80.0%-98.1%nucleotide homology with other published NDV strains." @default.
- W2351036694 created "2016-06-24" @default.
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- W2351036694 date "2008-01-01" @default.
- W2351036694 modified "2023-09-23" @default.
- W2351036694 title "Cloning and Characterization of Whole Genome of a Newcastle Disease Virus Strain from Chicken" @default.
- W2351036694 hasPublicationYear "2008" @default.
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