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- W2351042584 abstract "To investigate the telomerase activity in mesenchymal stem cells from rabbit bone marrow during their in vitro committed differentiation and its significance. Rabbit bone marrow was aspirated from the iliac crest sterilely with heparin. Bone marrow mononuclear cells were isolated by gradient density centrifugation with lymphocyte separation medium.Mesenchymal stem cells(MSCs) were then obtained and cultured in endothelial cell complete medium to differentiate into endothelial cells.The telomerase activity was analyzed during different periods by TRAPeze ELISA respectively. Telomerase activity was at lower level in freshly isolated bone marrow mononuclear cells and MSCs premultiplication. Once induced by vascular endothelial growth factor (VEGF), bone marrow MSCs were expanded potentially ex vivo , and their telomerase activity was soon upregulated and kept at a higher level in five passages. Telomerase activity observed in bone marrow MSCs expansion in vitro is a theoretical basis of clinical investigation on adult stem cell." @default.
- W2351042584 created "2016-06-24" @default.
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- W2351042584 date "2003-01-01" @default.
- W2351042584 modified "2023-09-25" @default.
- W2351042584 title "Telomerase activity during in vitro committed differentiation of rabbit bone marrow mesenchymal stem cells" @default.
- W2351042584 hasPublicationYear "2003" @default.
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