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- W2351059935 abstract "Gentamicin producing strain Micromonospora purpurea was treated with 15μg/ml penicillin when M.purpurea had cultured 36h,and the cells that had cultured for 48h at logarithmic growth phase were treated with UV and NTG,then they were cultured in incubator at 37℃ for 5～8 days,and when the colonies grew out,the cells were treated by UV again.As a result,a high yield strain N-14 was obtained.Compared to control,the biological titer of N-14 fermented in tube was increased from 625u/ml to 1913u/ml,and the biological titer of N-14 fermented in flask was 2178u/ml and increased by 75.2% compared to control 1243(u/ml).Besides,the reducing sugar,total sugar,amino nitrogen,pH,cell concentration and other metabolic parameters were determined during fermentation process in 5L fermentor,and the culture condition was researched.The average biological titer of 5 batches was 1967u/ml and was increased by 68% compared to control 1171u/ml." @default.
- W2351059935 created "2016-06-24" @default.
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- W2351059935 date "2011-04-26" @default.
- W2351059935 modified "2023-09-27" @default.
- W2351059935 title "Study on Superimposed Mutation on Micromonospora purpurea and Fermentation Process in 5L Fermentor" @default.
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