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- W2351124879 abstract "Screened the gonad transcriptome and used RT-PCR method,a metallothionein gene was amplified from Hyriopsis schlegelii.It contains 216bp and encodes 71aa.The deduced amino acids sequence was rich in cysteine and Cys-X(1-3)-Cys structure which was classic structure in MT genes.Multiple alignment showed high identity of MT gene from different species and shared 100% similarity with Hyriopsis cumingii.Then the fragment was subcloned into the expression vector pET-32aand was expressed in E.coli BL21(DE3)cells.The results of SDS-PAGE showed that the fusion protein was highly expressed after inducing by 1mM IPTG at 4hours and its molecular mass was about 27kDa.The recombinant MT protein were mainly soluble and was successfully purified by affinity chromatography.The proliferation experiment showed that the purified MT fusion protein had biological activities which could effectively protect cervix cancer Hela cells from oxidative stress." @default.
- W2351124879 created "2016-06-24" @default.
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- W2351124879 date "2014-01-01" @default.
- W2351124879 modified "2023-09-23" @default.
- W2351124879 title "Prokaryotic expression and activity analysis of metallothionein from Hyriopsis schlegelii" @default.
- W2351124879 hasPublicationYear "2014" @default.
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