FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2351142367> ?p ?o ?g. }

• W2351142367 endingPage "S299" @default.
• W2351142367 startingPage "S299.4" @default.
• W2351142367 abstract "Purpose To examine the role of potentially harmful cytokines, specifically IL-6, in renal damage that occurs during cold storage and rewarming of kidneys or tubular cells in vitro or immediately after kidney transplantation. Methods The in vitro model of renal cell injury used RPTE cells stored in University of Wisconsin solution (UW) at 4°C and returned to 37°C in standard conditions, mimicking the storage of cadaveric kidneys. To examine the effect of cytokines on RPTE cells, human TNF-α, IL-1α and -β, and IL-6 were used at 10 ng/mL. Cell injury was measured by LDH release. A Furth-Wistar rat in vivo renal injury model was used and is similar to the manner in which human kidneys are transplanted, using 24 hr of cold storage in UW. Rat IL-6 was measured by sandwich enzyme linked immunsorbent assay in the serum and urine of native and transplanted kidneys and from an effluent from the kidneys prior to and after cold storage. Results Using a TransSignal RayBio Human Cytokine Antibody Array-3 to screen for inflammatory mediator production, we found that IL-6 was present in small amounts in normally cultured cells and significantly upregulated at 24 hr after 24 hr of cold storage of RPTE cells. IL-8 and MCP-1 were moderately upregulated, and VEGF and IL-15 were slightly uprgulated. We focused on IL-6 because of its apparent constitutive production, significant upregulation, and potentially harmful effects. IL-6 has not previously been reported to be produced from renal tubular cells. Using typical inducers of IL-6, IL-1α/β was found to be the best inducer of IL-6 in RPTE cells. Large amounts were also induced by bacterial lipopolysaccharide (1 μg/mL), TNF-α, and phorbol ester (10 ng/mL) with a calcium ionophore (A23187, 100 ng/mL). In in vitro cell injury experiments RPTE cells cold stored and returned to normal 37°C culture conditions produced large amounts of IL-6 24 to 48 hrs later, as identified on the cytokine array. By LDH release, IL-6 did not induce cell death at 37°C but did greatly enhanced cold-rewarming induced cell death. Deferoxamine, an antioxidant that protects these cells from cold-rewarming injury, slightly reduced basal RPTE cell IL-6, but not cold-rewarming induced IL-6. In two in vivo rat transplant experiments, we have found that the urine or ex vivo effluent from a transplanted kidney produced more IL-6 than the native kidney. Conclusions RPTE cells have the ability to produce IL-6 in relatively large amounts. Rewarming renal tubular cells after cold storage and reperfusion of a cold stored rat kidney by transplantation also induces IL-6. We conclude that local IL-6 production may play a role in renal damage after cold storage." @default.
• W2351142367 created "2016-06-24" @default.
• W2351142367 creator A5042481555 @default.
• W2351142367 creator A5051845397 @default.
• W2351142367 creator A5053380066 @default.
• W2351142367 creator A5084910776 @default.
• W2351142367 date "2005-01-01" @default.
• W2351142367 modified "2023-09-26" @default.
• W2351142367 title "262 INTERLEUKIN-6 IN HUMAN RENAL PROXIMAL TUBULAR CELL DAMAGE IN COLD STORAGE AND REWARMING AND IN TRANSPLANTATION" @default.
• W2351142367 doi "https://doi.org/10.2310/6650.2005.00006.261" @default.
• W2351142367 hasPublicationYear "2005" @default.
• W2351142367 type Work @default.
• W2351142367 sameAs 2351142367 @default.
• W2351142367 citedByCount "0" @default.
• W2351142367 crossrefType "journal-article" @default.
• W2351142367 hasAuthorship W2351142367A5042481555 @default.
• W2351142367 hasAuthorship W2351142367A5051845397 @default.
• W2351142367 hasAuthorship W2351142367A5053380066 @default.
• W2351142367 hasAuthorship W2351142367A5084910776 @default.
• W2351142367 hasConcept C126322002 @default.
• W2351142367 hasConcept C144027150 @default.
• W2351142367 hasConcept C16685009 @default.
• W2351142367 hasConcept C2911091166 @default.
• W2351142367 hasConcept C2988529969 @default.
• W2351142367 hasConcept C2992666275 @default.
• W2351142367 hasConcept C71924100 @default.
• W2351142367 hasConcept C86803240 @default.
• W2351142367 hasConceptScore W2351142367C126322002 @default.
• W2351142367 hasConceptScore W2351142367C144027150 @default.
• W2351142367 hasConceptScore W2351142367C16685009 @default.
• W2351142367 hasConceptScore W2351142367C2911091166 @default.
• W2351142367 hasConceptScore W2351142367C2988529969 @default.
• W2351142367 hasConceptScore W2351142367C2992666275 @default.
• W2351142367 hasConceptScore W2351142367C71924100 @default.
• W2351142367 hasConceptScore W2351142367C86803240 @default.
• W2351142367 hasIssue "1" @default.
• W2351142367 hasLocation W23511423671 @default.
• W2351142367 hasOpenAccess W2351142367 @default.
• W2351142367 hasPrimaryLocation W23511423671 @default.
• W2351142367 hasRelatedWork W1968357221 @default.
• W2351142367 hasRelatedWork W2012485414 @default.
• W2351142367 hasRelatedWork W2035178996 @default.
• W2351142367 hasRelatedWork W2080658585 @default.
• W2351142367 hasRelatedWork W2342925211 @default.
• W2351142367 hasRelatedWork W2587860570 @default.
• W2351142367 hasRelatedWork W2963137190 @default.
• W2351142367 hasRelatedWork W4205692301 @default.
• W2351142367 hasRelatedWork W4233492667 @default.
• W2351142367 hasRelatedWork W4235209591 @default.
• W2351142367 hasVolume "53" @default.
• W2351142367 isParatext "false" @default.
• W2351142367 isRetracted "false" @default.
• W2351142367 magId "2351142367" @default.
• W2351142367 workType "article" @default.