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- W2351147475 abstract "Objective To investigate the surface marker expression and the differentiation capacity of adipose stem cells(ADSCs) when induced into adipocytes,osteoblasts,and neurons.Methods Inguinal,epididymis and post peritoneal fat tissues from rats were minced and digested with collagenase type I to isolate ADSCs,which were then cultivated in media.At confluence,the cells were passed.The third passage was identified by CD 29,CD 44,and CD 45.The cells were either cultivated in osteoblast-inducing,adipocytes-inducing or neurons-inducing culture medium,and alizarin red staining,oil red staining,or immunofluorescence were used to determine the outcome,respectively.Results ADSC expressed CD29(99.07±0.12)% and CD44(95.82±0.68)% at high levels and expressd CD45at low levels(0.11±0.12)%.Red calcified tubercles in the center of the gathered cells and prunosus particles in the plasm were observed through alizarin red staining and alkali ne phosphatase(ALP),respectively,4 weeks after osteoblast induction.Accumulation of salmon pink lipid drops was detected by oil red O one week after adipocytes induction.Nestin,NF-200,and GFAP were expressed 6 days after neuron induction.Conclusion ADSC expresses surface markers of.mesenchyme cells and can be induced into adipocytes,osteoblasts,and neurons in vitro,indicating ADSC has a multilineage differentiation capability" @default.
- W2351147475 created "2016-06-24" @default.
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- W2351147475 date "2011-01-01" @default.
- W2351147475 modified "2023-09-27" @default.
- W2351147475 title "Differentiation of Adipose Stem Cells into Adipocytes,Osteoblasts and Neurons in Vitro" @default.
- W2351147475 hasPublicationYear "2011" @default.
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