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- W2351153285 abstract "To express E protein of Duck tembusu virus(DTMUV),E gene was optimized according to the bias codon usages of insect cells.The optimized E gene(opti-E) was cloned into the multiple cloning site of pFastBacHT vector of the Bac-to-Bac expression system.The recombinant plasmid pFastBac-optiE was then transformed into DH10Bac for blue/white selection and antibiotics selection.The positive recombinant bacmid rBacmid-optiE was transfected to Sf9 in logarithmic growth phase for harvesting recombinant baculovirus.The E protein expressed in Sf9 was confirmed in Western blot and IFA.The expression of E protein optimized was based for the further study of its structure and its function." @default.
- W2351153285 created "2016-06-24" @default.
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- W2351153285 date "2013-01-01" @default.
- W2351153285 modified "2023-09-26" @default.
- W2351153285 title "EXPRESSION OF DUCK TEMBUSU VIRUS E GENE IN INSECT CELLS WITH CONDON OPTIMIZATION" @default.
- W2351153285 hasPublicationYear "2013" @default.
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