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- W2351198011 abstract "OBJECTIVE A method was developed for the determination of quercetin (Q) and kaempferol ( K) in rabbit plasma.METHODS Plasma samples were treated with acid hydrolyzation and ether extraction, the organic layer was evaporated to dryness, the residues were reconstituted in 100μL mobile phase prior to injection onto the high-performance liquid chr omatography(HPLC). A YWG C 18 column was used with a mobile phase of 0.0 1 mol·L -1 phosphate buffer(pH 2)-tetrahydrofuran-me thanol-isopropynol (60∶15∶10∶ 15). The elution was performed at the flow rate of 0.5 mL· min -1,detected at 380 nm.RESULTS There was a good linear relationship over the concentration range of 16.66 - 156.8 μg·L -1 for quercetin and kaempferol with coefficient of 0.991 2 and 0.994 1 respectively. Limits of detection were 4.2 μg·L -1 for quercetin and kaempferol. The average recoveries of the method were 101.0 % with RSD of 5.05%, and 103.0% with RSD 4.72% for quercetin and kaempferol, respectively.CONCLUSION This method is simple and accurate, it could be applied to the determination of quercetin and kaempferol in rabbit plasma after oral administration of Ginkgo bilaoba extract." @default.
- W2351198011 created "2016-06-24" @default.
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- W2351198011 date "2005-01-01" @default.
- W2351198011 modified "2023-09-23" @default.
- W2351198011 title "Determination of quercetin and kaempferal in rabbit plasma by HPLC" @default.
- W2351198011 hasPublicationYear "2005" @default.
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