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- W2351246375 abstract "Objective:Expression and characterization of recombinant ScFv antibody detecting P-glycoprotein.Methods:Amplify the gene of variable region of heavy chain(VH) and light chain (VL) of the antibody against P-glycoprotein from hybridoma cell line PHMA02. VH and VL genes were joined as single chain Fv (ScFv) by a flexible peptide linker and cloned into the plasmid PET28a(+) to construct the ScFv expression vector PET28a(+)-PgpScFv,and expressed from E.coli.Results:The anti-Pgp ScFv was expressed,isolated and purified. Like the native Mab PHMA02,the recombinant ScFv showed specific binding activity for the resistant cell line K562/A02 ,but can not block the activity of Pgp." @default.
- W2351246375 created "2016-06-24" @default.
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- W2351246375 date "2004-01-01" @default.
- W2351246375 modified "2023-09-25" @default.
- W2351246375 title "Expression and characterization of recombinant ScFv antibody detecting p-glycoprotein" @default.
- W2351246375 hasPublicationYear "2004" @default.
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