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- W2351274456 abstract "【Objective】 To observe the effect of insulin on PPARγ2 mRNA expression in 3T3-L1 cells with human recombinant Adiponectin.【Methods】 After the 3T3-L1 cells and 3T3-L1 cells with plasmid and 3T3-L1 cells with human recombinant Adiponectin incubated with insulin(500 ng/mL) for 24 h,cells were collected,total RNA were extracted.The PPARγ2 mRNA expression were quantitated by semi-quantitative RT-PCR.【Results】 Compared to controls the 3T3-L1 cells and the 3T3-L1 cells with plasmid,the PPAR-γ2 mRNA expression of 3T3-L1 celles with human recombinant Adiponectin was higher(P 0.01).In the 3T3-L1 cells and 3T3-L1 cells with plasmids and 3T3-L1 cells with human recombinant Adiponectin,treatment of 3T3-L1 cells by insulin decreased PPARγ2 mRNA expression compared to untreated controls(P 0.05).Effect of insulin on PPARγ2 mRNA expression in 3T3-L1 cells was reversed by stably transfected human recombinant Adiponectin.【Conclusions】 The 3T3-L1 cells stably transfected human recombinant Adiponectin increased PPARγ2 mRNA expression in the 3T3-L1 cells compared to controls.Insulin suppressed PPARγ2 mRNA expression in the 3T3-L1 cells.Effect of insulin on PPARγ2 mRNA expression in 3T3-L1 cells was reversed by stably transfected human recombinant Adiponectin." @default.
- W2351274456 created "2016-06-24" @default.
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- W2351274456 date "2011-01-01" @default.
- W2351274456 modified "2023-09-24" @default.
- W2351274456 title "Effect of PPARγ2 mRNA expression in 3T3-L1 Adipocytes with the human recombinant Adiponectin by insulin" @default.
- W2351274456 hasPublicationYear "2011" @default.
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