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- W2351294564 abstract "Bacillus subtilis protease apr gene was cloned by PCR technology,the homology of this gene and code protein were compared,which enzymology characteristic was analyzed.It showed that full-length of gene sequence was 1 509 bp,503 amino acid being coded,and the consistency of sequencing results was 100%.Recombinant expression plasmid pET-22b(+)-apr was constructed and expressed in E.coli BL21 successfully,protein molecular weight was 55 ku,and the determined enzyme activity was 19 500 U·mL-1.The cloning and analysis of this gene could provide valuable gene material for small peptide preparing of soybean protein,which had great significance on deep studying of the molecular biologicalmechanism." @default.
- W2351294564 created "2016-06-24" @default.
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- W2351294564 date "2010-01-01" @default.
- W2351294564 modified "2023-09-25" @default.
- W2351294564 title "Clone and expression of Bacillus subtilis apr gene" @default.
- W2351294564 hasPublicationYear "2010" @default.
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