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- W2351320005 abstract "Objective:To clone, sequence and analyse the homology of sequences of five arboviruses (MAY, SFV, MVE, ILH and BUN) which belong to Togaviridae, Flaviviridae or Bunyaviridae,respectively.Methods:RNAs of five arboviruses were extracted from infected sucking mice brains. With the RNAs as templates, cDNA fragments containing the regions encoding structure or non_structure protein were obtained by reverse transcription polymerase chain reaction (RT_PCR). The PCR products were cloned into pGEM_T_Easy vector and sequenced. The nucleotide sequences of the five arboviruses were compared with the sequences in GenBank and their homology was analysed.Results:Sequence homology analyses revealed that five arboviruses had the highest homology with their corresponding sequences in GenBank. The corresponding results of homology analyses were MAY 97%,SFV 99%,MVE 99%,ILH 99% and BUN 98%.Conclusion:The five arboviruses were identified by the nucleotide sequence and comparative analysis." @default.
- W2351320005 created "2016-06-24" @default.
- W2351320005 creator A5084290427 @default.
- W2351320005 date "2002-01-01" @default.
- W2351320005 modified "2023-09-25" @default.
- W2351320005 title "Part nucleotide sequence determination and homology analysis of five arbovirus genomes" @default.
- W2351320005 hasPublicationYear "2002" @default.
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