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- W2351365281 abstract "The prokaryotic expression vector for gene encoding the fusion protein HCTV [adhesion A of H.pylori(hpaA),B-segment of the vacuolating cytotoxin of H.pylori(VacA),A-subunit of cholera enterotoxin(Ctx-B)] was constructed and expressed to lay a foundation for prophylaxis and treatment of H.pylori infection.For this purpose,the target genes for hpaA and CtxB were amplified from plasmid pQE-hcB with PCR technique,and VacA gene was amplified from plasmid pQE-30-V. These genes were inserted into prokaryotic expression vector pQE-30 to construct pQE-hctv,and then transformed into E.coli DH5α cells.The gene products were expressed after IPTG induction,as the fusion protein HCTV and analyzed by SDS-PAGE.Its immunogencity was identified by Western blotting.It was found that the relative molecular weight of this fusion protein was approximately about 68000 with the purity of more than 90% as demonstrated by affinity chromatography.This fusion protein could be specifically recognized by anti-HpaA,anti-VacA and anti CT antisera.It is concluded that the recombinant expression plasmid pQE-30-hctv was successfully constructed and expressed with definite immunogenicity and specificity,thus providing the basis for the candidate antigen to be used in the oral vaccine against H.pylori infection." @default.
- W2351365281 created "2016-06-24" @default.
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- W2351365281 date "2006-01-01" @default.
- W2351365281 modified "2023-09-24" @default.
- W2351365281 title "Preparation and immunogenicity study on the fusion protein HCTV of Helicobacter pylori" @default.
- W2351365281 hasPublicationYear "2006" @default.
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