Matches in SemOpenAlex for { <https://semopenalex.org/work/W2351386319> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2351386319 abstract "This study was designed to construct non-structural protein NS1 eukaryotic expression vector of influenza A virus subtype H7N9 and express the NS1 protein in 293 Tcells.The NS1 gene of influenza A virus subtype H7N9 was amplified by RT-PCR and cloned into pcDNA4-Flag-HA vector to construct a plasmid,named pcDNA4-Flag-HA-NS1.The recombinant eukaryotic expression vector pcDNA4-Flag-HA-NS1 subsequently yielded.The expression of the NS1 gene in transfected 293 T cells was tested by Western blotting.The results showed that the recombinant eukaryotic expression vector pcDNA4-Flag-HANS1 was successfully constructed;the NS1 protein was finally expressed in 293T cells;the full-length NS1 gene was obtained as well as its recombinant eukaryotic expression plasmid was successfully constructed and expressed in 293 Tcells.The construction of eukaryotic expression plasmid of NS1 gene was made,and made foundations for further study the function of NS1 protein and the mechanism of diseases induced by influenza A virus." @default.
- W2351386319 created "2016-06-24" @default.
- W2351386319 creator A5018889723 @default.
- W2351386319 date "2014-01-01" @default.
- W2351386319 modified "2023-10-02" @default.
- W2351386319 title "Construction and Expression of NS1 Protein Eukaryotic Expression Vector of Influenza A Virus Subtype H7N9" @default.
- W2351386319 hasPublicationYear "2014" @default.
- W2351386319 type Work @default.
- W2351386319 sameAs 2351386319 @default.
- W2351386319 citedByCount "0" @default.
- W2351386319 crossrefType "journal-article" @default.
- W2351386319 hasAuthorship W2351386319A5018889723 @default.
- W2351386319 hasConcept C104317684 @default.
- W2351386319 hasConcept C123894998 @default.
- W2351386319 hasConcept C150194340 @default.
- W2351386319 hasConcept C153911025 @default.
- W2351386319 hasConcept C159047783 @default.
- W2351386319 hasConcept C22744801 @default.
- W2351386319 hasConcept C2522874641 @default.
- W2351386319 hasConcept C2777546802 @default.
- W2351386319 hasConcept C30417494 @default.
- W2351386319 hasConcept C33161422 @default.
- W2351386319 hasConcept C40767141 @default.
- W2351386319 hasConcept C54009773 @default.
- W2351386319 hasConcept C54355233 @default.
- W2351386319 hasConcept C86803240 @default.
- W2351386319 hasConcept C92087593 @default.
- W2351386319 hasConceptScore W2351386319C104317684 @default.
- W2351386319 hasConceptScore W2351386319C123894998 @default.
- W2351386319 hasConceptScore W2351386319C150194340 @default.
- W2351386319 hasConceptScore W2351386319C153911025 @default.
- W2351386319 hasConceptScore W2351386319C159047783 @default.
- W2351386319 hasConceptScore W2351386319C22744801 @default.
- W2351386319 hasConceptScore W2351386319C2522874641 @default.
- W2351386319 hasConceptScore W2351386319C2777546802 @default.
- W2351386319 hasConceptScore W2351386319C30417494 @default.
- W2351386319 hasConceptScore W2351386319C33161422 @default.
- W2351386319 hasConceptScore W2351386319C40767141 @default.
- W2351386319 hasConceptScore W2351386319C54009773 @default.
- W2351386319 hasConceptScore W2351386319C54355233 @default.
- W2351386319 hasConceptScore W2351386319C86803240 @default.
- W2351386319 hasConceptScore W2351386319C92087593 @default.
- W2351386319 hasLocation W23513863191 @default.
- W2351386319 hasOpenAccess W2351386319 @default.
- W2351386319 hasPrimaryLocation W23513863191 @default.
- W2351386319 hasRelatedWork W2176024459 @default.
- W2351386319 hasRelatedWork W2347229418 @default.
- W2351386319 hasRelatedWork W2350753429 @default.
- W2351386319 hasRelatedWork W2351780042 @default.
- W2351386319 hasRelatedWork W2354165814 @default.
- W2351386319 hasRelatedWork W2355546949 @default.
- W2351386319 hasRelatedWork W2359240458 @default.
- W2351386319 hasRelatedWork W2361293435 @default.
- W2351386319 hasRelatedWork W2365999384 @default.
- W2351386319 hasRelatedWork W2367391397 @default.
- W2351386319 hasRelatedWork W2372976052 @default.
- W2351386319 hasRelatedWork W2373774617 @default.
- W2351386319 hasRelatedWork W2380974657 @default.
- W2351386319 hasRelatedWork W2388859550 @default.
- W2351386319 hasRelatedWork W2389035195 @default.
- W2351386319 hasRelatedWork W2389257601 @default.
- W2351386319 hasRelatedWork W2390823609 @default.
- W2351386319 hasRelatedWork W2391899646 @default.
- W2351386319 hasRelatedWork W2393929562 @default.
- W2351386319 hasRelatedWork W2394189279 @default.
- W2351386319 isParatext "false" @default.
- W2351386319 isRetracted "false" @default.
- W2351386319 magId "2351386319" @default.
- W2351386319 workType "article" @default.