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- W2351483896 abstract "Objective To construct the RNA interference(RNAi)expression vector of Ku70 gene and evaluate the effect of RNAi.Methods Three small interfering RNA(siRNA) targeting Ku70 was designed for transfecting Hela cell line.Through immunoblotting assay,the expression of Ku70 was examined at 24,48 and 72 hours after transfection into Hela cell line.The siRNA which have the most effect of RNAi was designed into short-hairpin RNA and cloned into pSilencerTM 4.1-CMV-hygro vector,then transfection Hela cell line and the stable strain were selected.The interference effects of Ku70 siRNA was evaluated at mRNA and protein levels.Results The expression of Ku70 was not obviously changed at 24 and 48 hours after siRNA transfected Hela cells,but was significantly down-regulated at 72 hours.At the stable transfection strain which with stable expression of siRNA,the expression of Ku70 gene was inhibited apparentely at mRNA and protein levels.Conclusion The RNAi vector targeting Ku70 gene is successfully constructed,and the best efficacy series are selected,which lays solid foundation for further study of the relationship between Ku70 gene expression and the cervical cancer susceptibility with radiation therapy." @default.
- W2351483896 created "2016-06-24" @default.
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- W2351483896 date "2011-01-01" @default.
- W2351483896 modified "2023-09-23" @default.
- W2351483896 title "Construction and assessment of effects of Ku70 RNA interference vector" @default.
- W2351483896 hasPublicationYear "2011" @default.
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