FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2351503164> ?p ?o ?g. }

• W2351503164 endingPage "764" @default.
• W2351503164 startingPage "754" @default.
• W2351503164 abstract "Skin substitutes significantly reduce the morbidity and mortality of patients with burn injuries and chronic wounds. However, current skin substitutes have disadvantages related to high costs and inadequate skin regeneration due to highly inflammatory wounds. Thus, new skin substitutes are needed. By combining two polymers, pullulan, an inexpensive polysaccharide with antioxidant properties, and gelatin, a derivative of collagen with high water absorbency, we created a novel inexpensive hydrogel-named PG-1 for pullulan-gelatin first generation hydrogel-suitable for skin substitutes. After incorporating human fibroblasts and keratinocytes onto PG-1 using centrifugation over 5 days, we created a cellularized bilayer skin substitute. Cellularized PG-1 was compared to acellular PG-1 and no hydrogel (control) in vivo in a mouse excisional skin biopsy model using newly developed dome inserts to house the skin substitutes and prevent mouse skin contraction during wound healing. PG-1 had an average pore size of 61.69 μm with an ideal elastic modulus, swelling behavior, and biodegradability for use as a hydrogel for skin substitutes. Excellent skin cell viability, proliferation, differentiation, and morphology were visualized through live/dead assays, 5-bromo-2'-deoxyuridine proliferation assays, and confocal microscopy. Trichrome and immunohistochemical staining of excisional wounds treated with the cellularized skin substitute revealed thicker newly formed skin with a higher proportion of actively proliferating cells and incorporation of human cells compared to acellular PG-1 or control. Excisional wounds treated with acellular or cellularized hydrogels showed significantly less macrophage infiltration and increased angiogenesis 14 days post skin biopsy compared to control. These results show that PG-1 has ideal mechanical characteristics and allows ideal cellular characteristics. In vivo evidence suggests that cellularized PG-1 promotes skin regeneration and may help promote wound healing in highly inflammatory wounds, such as burns and chronic wounds." @default.
• W2351503164 created "2016-06-24" @default.
• W2351503164 creator A5003138012 @default.
• W2351503164 creator A5011495800 @default.
• W2351503164 creator A5038229829 @default.
• W2351503164 date "2016-05-01" @default.
• W2351503164 modified "2023-10-01" @default.
• W2351503164 title "Cellularized Bilayer Pullulan-Gelatin Hydrogel for Skin Regeneration" @default.
• W2351503164 cites W1549118871 @default.
• W2351503164 cites W1949832432 @default.
• W2351503164 cites W1978011501 @default.
• W2351503164 cites W1984693616 @default.
• W2351503164 cites W1986161286 @default.
• W2351503164 cites W1987482585 @default.
• W2351503164 cites W1997327997 @default.
• W2351503164 cites W1997525542 @default.
• W2351503164 cites W2000540654 @default.
• W2351503164 cites W2005548107 @default.
• W2351503164 cites W2006469915 @default.
• W2351503164 cites W2010114799 @default.
• W2351503164 cites W2010337583 @default.
• W2351503164 cites W2021355758 @default.
• W2351503164 cites W2022397532 @default.
• W2351503164 cites W2030160629 @default.
• W2351503164 cites W2032554052 @default.
• W2351503164 cites W2038403078 @default.
• W2351503164 cites W2039657481 @default.
• W2351503164 cites W2042709468 @default.
• W2351503164 cites W2045232408 @default.
• W2351503164 cites W2053585356 @default.
• W2351503164 cites W2055367142 @default.
• W2351503164 cites W2056868690 @default.
• W2351503164 cites W2057878147 @default.
• W2351503164 cites W2061761744 @default.
• W2351503164 cites W2070942486 @default.
• W2351503164 cites W2076027912 @default.
• W2351503164 cites W2081925937 @default.
• W2351503164 cites W2085293124 @default.
• W2351503164 cites W2089384070 @default.
• W2351503164 cites W2092535047 @default.
• W2351503164 cites W2100338747 @default.
• W2351503164 cites W2102981308 @default.
• W2351503164 cites W2105854283 @default.
• W2351503164 cites W2119988457 @default.
• W2351503164 cites W2131836314 @default.
• W2351503164 cites W2133147905 @default.
• W2351503164 cites W2137652101 @default.
• W2351503164 cites W2140327368 @default.
• W2351503164 cites W2143543459 @default.
• W2351503164 cites W2145169153 @default.
• W2351503164 cites W2148028643 @default.
• W2351503164 cites W2148383254 @default.
• W2351503164 cites W2153320120 @default.
• W2351503164 cites W2165269428 @default.
• W2351503164 cites W2166690473 @default.
• W2351503164 cites W2362472408 @default.
• W2351503164 cites W267982658 @default.
• W2351503164 cites W269626472 @default.
• W2351503164 doi "https://doi.org/10.1089/ten.tea.2015.0536" @default.
• W2351503164 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4876533" @default.
• W2351503164 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27072720" @default.
• W2351503164 hasPublicationYear "2016" @default.
• W2351503164 type Work @default.
• W2351503164 sameAs 2351503164 @default.
• W2351503164 citedByCount "51" @default.
• W2351503164 countsByYear W23515031642016 @default.
• W2351503164 countsByYear W23515031642017 @default.
• W2351503164 countsByYear W23515031642018 @default.
• W2351503164 countsByYear W23515031642019 @default.
• W2351503164 countsByYear W23515031642020 @default.
• W2351503164 countsByYear W23515031642021 @default.
• W2351503164 countsByYear W23515031642022 @default.
• W2351503164 countsByYear W23515031642023 @default.
• W2351503164 crossrefType "journal-article" @default.
• W2351503164 hasAuthorship W2351503164A5003138012 @default.
• W2351503164 hasAuthorship W2351503164A5011495800 @default.
• W2351503164 hasAuthorship W2351503164A5038229829 @default.
• W2351503164 hasBestOaLocation W23515031642 @default.
• W2351503164 hasConcept C100817775 @default.
• W2351503164 hasConcept C108586683 @default.
• W2351503164 hasConcept C136229726 @default.
• W2351503164 hasConcept C141071460 @default.
• W2351503164 hasConcept C171056886 @default.
• W2351503164 hasConcept C185592680 @default.
• W2351503164 hasConcept C188027245 @default.
• W2351503164 hasConcept C190567982 @default.
• W2351503164 hasConcept C192562407 @default.
• W2351503164 hasConcept C2777459323 @default.
• W2351503164 hasConcept C2778823387 @default.
• W2351503164 hasConcept C2779257994 @default.
• W2351503164 hasConcept C2780269544 @default.
• W2351503164 hasConcept C49892992 @default.
• W2351503164 hasConcept C54355233 @default.
• W2351503164 hasConcept C55493867 @default.
• W2351503164 hasConcept C71924100 @default.
• W2351503164 hasConcept C86803240 @default.
• W2351503164 hasConcept C95444343 @default.
• W2351503164 hasConceptScore W2351503164C100817775 @default.

• next