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- W2351511916 abstract "Objective To explore the optimal SENV DNA isolation and primers to develop a sensitive ,rapid and specific polymerase chain reaction for the detection of SEN virus Methods Acupure DNA/RNA, SDS proteinase K and distinctive TTV DNA isolation procedures were used for isolation the viral genome, and three groups primers were used to amplify SENV DNA, the influence of different sera isolation method, amplification primers and the sample volume for the positive detection rate of SEN virus were compared Results Among 191 samples, 15, 9 and 7 samples were positive in Acupure DNA/RNA isolation, proteinase K isolation and distinctive TTV DNA isolation respectively The positive PCR result of two nest PCR primers were 15 and 11, only 2 samples were detected with one time PCR and at least 50μl of sample was needed when used primers group 2 Conclusions Different sera isolation method and amplification primers influence the positive detection rate of SEN virus" @default.
- W2351511916 created "2016-06-24" @default.
- W2351511916 creator A5011454202 @default.
- W2351511916 date "2005-01-01" @default.
- W2351511916 modified "2023-09-23" @default.
- W2351511916 title "Optimal condition of polymerase chain reaction for detection SEN virus DNA" @default.
- W2351511916 hasPublicationYear "2005" @default.
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