Matches in SemOpenAlex for { <https://semopenalex.org/work/W2351589443> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W2351589443 endingPage "30" @default.
- W2351589443 startingPage "27" @default.
- W2351589443 abstract "To construct the RNA interference expression vector for expression of human neuropathy target esterase (NTE) gene in mammalian cells.Spe I and Xho I-digested insert from pSUPER, which comprised H1 RNA polymerase III promoter and the multiple cloning sites, were cloned into the compatible in the pcDNA3.1 (+) to generate pSUPER/neo that could express small interfering RNA in mammalian cells. The annealed oligos targeting the expression of NTE were ligated into pSUPER/neo vector digested with Bgl II and Hind III to generate pSUPER/neo-NTE, which was transfected into COS7 and SH-SY5Y cells. The inhibitory effect of the expression of NTE was detected by western blot analysis and the enzyme activity assay.pSUPER/neo-NTE could stably express double-stranded RNA of NTE. The expression of pSUPER/neo-NTE in COS7 and SH-SY5Y cells could efficiently inhibit the activity of NTE in the mammalian cells.Stable eukaryotic expression vector of double-stranded RNA of NTE, pSUPER/neo-NTE, has been constructed successfully with promoter substitution strategy." @default.
- W2351589443 created "2016-06-24" @default.
- W2351589443 creator A5000432967 @default.
- W2351589443 creator A5053453125 @default.
- W2351589443 creator A5080681903 @default.
- W2351589443 creator A5090732781 @default.
- W2351589443 date "2006-01-01" @default.
- W2351589443 modified "2023-09-22" @default.
- W2351589443 title "[Construction of RNA interference expression vectors of human neuropathy target esterase and its inhibition for expression of NTE in mammalian cells]." @default.
- W2351589443 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16600090" @default.
- W2351589443 hasPublicationYear "2006" @default.
- W2351589443 type Work @default.
- W2351589443 sameAs 2351589443 @default.
- W2351589443 citedByCount "0" @default.
- W2351589443 crossrefType "journal-article" @default.
- W2351589443 hasAuthorship W2351589443A5000432967 @default.
- W2351589443 hasAuthorship W2351589443A5053453125 @default.
- W2351589443 hasAuthorship W2351589443A5080681903 @default.
- W2351589443 hasAuthorship W2351589443A5090732781 @default.
- W2351589443 hasConcept C104317684 @default.
- W2351589443 hasConcept C105580179 @default.
- W2351589443 hasConcept C153911025 @default.
- W2351589443 hasConcept C166703698 @default.
- W2351589443 hasConcept C174510640 @default.
- W2351589443 hasConcept C185592680 @default.
- W2351589443 hasConcept C190232843 @default.
- W2351589443 hasConcept C2776449523 @default.
- W2351589443 hasConcept C33161422 @default.
- W2351589443 hasConcept C40767141 @default.
- W2351589443 hasConcept C54009773 @default.
- W2351589443 hasConcept C55493867 @default.
- W2351589443 hasConcept C67705224 @default.
- W2351589443 hasConcept C70916841 @default.
- W2351589443 hasConcept C86803240 @default.
- W2351589443 hasConcept C95444343 @default.
- W2351589443 hasConceptScore W2351589443C104317684 @default.
- W2351589443 hasConceptScore W2351589443C105580179 @default.
- W2351589443 hasConceptScore W2351589443C153911025 @default.
- W2351589443 hasConceptScore W2351589443C166703698 @default.
- W2351589443 hasConceptScore W2351589443C174510640 @default.
- W2351589443 hasConceptScore W2351589443C185592680 @default.
- W2351589443 hasConceptScore W2351589443C190232843 @default.
- W2351589443 hasConceptScore W2351589443C2776449523 @default.
- W2351589443 hasConceptScore W2351589443C33161422 @default.
- W2351589443 hasConceptScore W2351589443C40767141 @default.
- W2351589443 hasConceptScore W2351589443C54009773 @default.
- W2351589443 hasConceptScore W2351589443C55493867 @default.
- W2351589443 hasConceptScore W2351589443C67705224 @default.
- W2351589443 hasConceptScore W2351589443C70916841 @default.
- W2351589443 hasConceptScore W2351589443C86803240 @default.
- W2351589443 hasConceptScore W2351589443C95444343 @default.
- W2351589443 hasIssue "1" @default.
- W2351589443 hasLocation W23515894431 @default.
- W2351589443 hasOpenAccess W2351589443 @default.
- W2351589443 hasPrimaryLocation W23515894431 @default.
- W2351589443 hasRelatedWork W1850936908 @default.
- W2351589443 hasRelatedWork W1999601787 @default.
- W2351589443 hasRelatedWork W2019886119 @default.
- W2351589443 hasRelatedWork W2103793964 @default.
- W2351589443 hasRelatedWork W2106975501 @default.
- W2351589443 hasRelatedWork W2115066994 @default.
- W2351589443 hasRelatedWork W2346786084 @default.
- W2351589443 hasRelatedWork W2351626879 @default.
- W2351589443 hasRelatedWork W2357504759 @default.
- W2351589443 hasRelatedWork W2363255534 @default.
- W2351589443 hasRelatedWork W2364188481 @default.
- W2351589443 hasRelatedWork W2365231899 @default.
- W2351589443 hasRelatedWork W2367969921 @default.
- W2351589443 hasRelatedWork W2373096866 @default.
- W2351589443 hasRelatedWork W2379470702 @default.
- W2351589443 hasRelatedWork W2386793111 @default.
- W2351589443 hasRelatedWork W2393324243 @default.
- W2351589443 hasRelatedWork W2397406539 @default.
- W2351589443 hasRelatedWork W2465476058 @default.
- W2351589443 hasRelatedWork W3017181318 @default.
- W2351589443 hasVolume "24" @default.
- W2351589443 isParatext "false" @default.
- W2351589443 isRetracted "false" @default.
- W2351589443 magId "2351589443" @default.
- W2351589443 workType "article" @default.