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- W2351608130 abstract "【Objective】 The present study was to investigate the effect of different sources of donor cells and TSA treatment on in vitro development of yak interspecies somatic cell nuclear transfer(iSCNT)embryos.【Method】 With cattle oocytes as recipient,yak fetal fibroblasts of 50-day-old,6-and 18-month-old adult fibroblasts as the donor nuclear,yak iSCNT embryos were reconstructed and then the effect of donor cell source on the development of cloned embryos evaluated.We compared the effect of different TSA treated concentrations(0,20,40,60 and 80 nmol/L)and times(0,6,8 and 12 h)on the development of yak cloned embryos,respectively,then determined the best program for treatment,and treated donor cells and embryos with this program for detecting the effect of TSA on the relative expression level of HDAC2 by qRT-PCR.【Result】 With fetal fibroblast cells as donor cells,yak interspecies cloning embryos blastocyst rate was the highest,but there was no significant difference(P0.05).Treatment donor cell with 40 nmol/L TSA for 6 h significantly increased the blastocyst rate(30.6%),and significant difference existed with the control group(P0.05).Treatment of reconstructed embryos with 40 nmol/L TSA for 12 h was helpful for embryos development.However,the blastocyst formation rate decreased along with the treated time and concentration of TSA.The expression level of HDAC2 was reduced significantly after donor cell and embryos treated with TSA.【Conclusion】 There was no significant effect of donor cells source on the development of yak iSCNT embryos.Treated donor cells for 6 h and reconstructed embryos for 12 h with 40 nmol/L TSA can significantly improve in vitro development of yak iSCNT embryos and reprogramming." @default.
- W2351608130 created "2016-06-24" @default.
- W2351608130 creator A5010324463 @default.
- W2351608130 date "2012-01-01" @default.
- W2351608130 modified "2023-09-22" @default.
- W2351608130 title "Effects of donor cell source and TSA treatment on reprogramming of yak-bovine iSCNT embryos" @default.
- W2351608130 hasPublicationYear "2012" @default.
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