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- W2351649444 abstract "[Objective] The aim was to provide the basis for genetic transformation of hygromycin resistance gene of Brassica napus L.[Method] With cotyledon and hypocotyl of B.napus varieties(Huyou 19,Suzhouqing,Wuyueman) as explants,the effects of treatments with different concn.of hygromycin on its browning rate and callus rate were studied.[Result] When hygromycin.was 3 mg/L,the browning rates of Wuyueman,Huyou 19 and Suzhouqing were 87.6%,30.7% and 40.1% resp.and the critical concentration.they browning was 6 mg/L.The tolerance of hypocotyl of Suzhouqing to hygromycin was stronger and the critical concentration of its browning was higher than that of Huyou 19 and Wuyueman.When hygromycin.was 6 mg/L,the callus rates of cotyledon of Huyou 19,Suzhouqing,Wuyueman were 3.7%,2.3% and 0 resp.and those of hypocotyl were 5.1%,8.1% and 2.2% resp.[Conclusion] When cotyledon of B.napus was taken as explants,the optimum screening concn.of 3 varieties on hygromycin was 6 mg/L,when its hypocotyl was taken as explants,the optimum screening concn.of Huyou 19 and Wuyueman on hygromycin was 6 mg/L and that of Suzhouqing was 9 mg/L." @default.
- W2351649444 created "2016-06-24" @default.
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- W2351649444 date "2009-01-01" @default.
- W2351649444 modified "2023-09-28" @default.
- W2351649444 title "Study on Sensitivity of Cotyledon and Hypocotyl of Brassica napus L.to Hygromycin" @default.
- W2351649444 hasPublicationYear "2009" @default.
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