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- W2351662704 abstract "OTA was linked to the carrier proteins BSA with NHS method to prepare the immunogen OTA-BSA,and linked to the carrier proteins OVA with EDC method to prepare the coating antigen OTA-OVA.UV scanning spectrum and SDS-PAGE preliminarily proved that the hapten OTA was coupled successfully to the carrier protein.The specific polyclonal antiserum with high titre were obtained by immunizing BALB/C mice with OTA-BSA of 10μg and 50μg each respectively,with the titre 1∶104 and the inhibition titer 14.7ng.These results indicated that OTA was successfully linked to the carrier proteins,and showed that a low dose of immunization can increase the sensitivity of pAb in the mouce.This study lay the foundation for further gain monoclonal anti-OTA and its immunological assay." @default.
- W2351662704 created "2016-06-24" @default.
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- W2351662704 date "2011-01-01" @default.
- W2351662704 modified "2023-09-23" @default.
- W2351662704 title "Synthesis and identification of ochratoxin A artificial immunogen" @default.
- W2351662704 hasPublicationYear "2011" @default.
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