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- W2351672143 abstract "To explore the feasibility of spermatozoa of Taihang goat as a vector of foreign genes to generate transgenic goats,and assess the effect of liposome during the spermatozoa transfection.Transfecting the spermatozoa by co-culture and liposome mediated methods with the FecB gene fragment.And two group samples were detected by PCR and FISH,and analyzed by statistics.The result showed that the spermatozoa could take exotic DNA voluntarily.The sperm positive rate of the total culture group was 18.3%;the sperm positive rate of liposome group was 38.7%.The transfection ratio of liposome mediated group was higher than co-culture group(P0.01).The spermatozoa of Taihang goat could be transfected with the FecB gene fragment mediated by liposome and provided the basis of transgenic tai hang goat by SMGT." @default.
- W2351672143 created "2016-06-24" @default.
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- W2351672143 date "2011-01-01" @default.
- W2351672143 modified "2023-10-04" @default.
- W2351672143 title "Experimental study on transgenic Taihang goat expressing FecB by sperm mediated gene transfer with liposome" @default.
- W2351672143 hasPublicationYear "2011" @default.
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