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• W2351693966 abstract "Objective To study the effect of p38MAPK activity on tumor necrosis factor-α (TNF-α) mRNA and intercellular adhesion molecule 1 (ICAM1) mRNA expressions of isolated rabbit liver during early stage of cold preservation and reperfusion period. Methods Based on the cold preservation and reperfusion model of isolated rabbit liver, the animals were divided into inhibition group (n=12) with 3 μmol/L SB202190 (p38MAPK specificity inhibitor) in perfusate and control group (n=12) with no SB202190 in perfusate. Liver tissue samples were harvested at the time points of before resection, end of cold preservation, and different reperfusion period (10, 30, 60 and 120 min). Protein expression and activity of p38MAPK were detected by Western blot and immunoprecipitation respectively, expression of TNF-α mRNA was detected by RT-PCR, and expression of ICAM1 mRNA was detected by in situ hybridization. Results There was no obvious change of expression of p38MAPK protein in liver tissue both in two groups during the total period (P0.05), and there was no statistically significant difference between two groups (P0.05). At time points of end of cold preservation, 10, 30 and 60 min of reperfusion, the activity of p38MAPK in control group was significantly higher than that at the time points of before resection and 120 min of reperfusion (P0.01), and was also significantly higher than that in inhibition group at the same time points (P0.01). There was no significant difference in activity of p38MAPK among all time points in inhibition group (P0.05). The expressions of TNF-α mRNA and ICAM1 mRNA at the time points of before resection, end of cold preservation, and 10 and 30 min of reperfusion were significantly lower than those in 60 and 120 min of reperfusion in both two groups (P0.05, P0.01); The expressions of TNF-α mRNA and ICAM1 mRNA in inhibition group were significantly lower than those in control group at the time points of 60 and 120 min of reperfusion (P0.01). The activity of p38MAPK of liver tissue during cold preservation and reperfusion period was significantly correlated with the level of TNF-α mRNA and level of ICAM1 mRNA expression (r=0.996, P0.01; r=0.985, P0.01). Conclusions These results suggest that p38MAPK pathway may regulate the expressions of TNF-α and ICAM1 at the level of transcription and the activation of p38MAPK can up-regulate TNF-α and ICAM1 expressions, which may be one of the important mechanisms to cause ischemia-reperfusion injury of isolated liver during cold preservation and reperfusion period." @default.
• W2351693966 created "2016-06-24" @default.
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• W2351693966 date "2009-01-01" @default.
• W2351693966 modified "2023-09-23" @default.
• W2351693966 title "Effect of p38MAPK Pathway on TNF-α mRNA and ICAM1 mRNA Expressions of Isolated Rabbit Liver Tissue During Early Stage of Cold Preservation and Reperfusion Period" @default.
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