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- W2351749446 abstract "Objective To evaluate the efficacy of immune responses to different kinds of recombinant HB(rHB) vaccines in different strains of mice.Metheods All the mice were injected HB vaccines in a serial dilution.Two NIH close-colonies of mice were typed in the H-2 region by using microcytotoxicity method and PCR.The titre of anti-HBsAg antibody was analyzed by microplate reading.The ED_50 was then estimated by Karder method for each strain.Results Significant differences were found between potency estimates derived from assays using the two close-colonies of NIH mice.Conclusion It is likely that the variation of immune responses to HB vaccines in mice is correlated with the H-2 haplotype.In some particular cases,the heterozygosity in H-2 region found in NIH stock may influence the accuracy in such testing even a reference preparation of HB vaccine was used.Based on our findings,attention should be paid to select an appropriate NIH stock with the H-2q haplotype for potency testing of HB vaccine in China." @default.
- W2351749446 created "2016-06-24" @default.
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- W2351749446 date "2009-01-01" @default.
- W2351749446 modified "2023-09-24" @default.
- W2351749446 title "Comparison of the Potency Testing by Immune Responses to Different Kinds of HB Vaccines in Two Close-Colonies of NIH Mice" @default.
- W2351749446 hasPublicationYear "2009" @default.
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