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- W2351776761 abstract "To study the hemolytic gene or main gene causing hemolysis in Bacillus subtilis 224(BS224),the paper took BS224 genomic DNA as templates,realized the amplification of two DNA sequences by using the PCR method,namely about the yplQ gene upstream 1.0 kb and downstream 0.5 kb of yplQ gene,then set the recombinant plasmid pMD18-T-neo with insertion of the expression unit of a neomycin resistance gene as skeleton,to construct a gene blocking plasmid pMD18-T-neo-yplQ based on yplQ loci.And BS224 after linearization and electrotransformation pMD18-T-neo-yplQ,and obtain 36 neor resistance transformant through new mycin resistance plate.One strain named yplQ18 was identified to be yplQ disruptant by PCR identification and nucleotide sequence evidence.Meanwhile,the test inoculated it to 5% sheep blood agar plate for hemolytic test,and it still caused hemolysis.The results showed that it played no effects or few effects on hemolytic of bacterial strain to solely knockout yplQ gene from BS224." @default.
- W2351776761 created "2016-06-24" @default.
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- W2351776761 date "2010-01-01" @default.
- W2351776761 modified "2023-09-22" @default.
- W2351776761 title "Knockout of yplQ gene in Bacillus subtilis 224 and influence on hemolysis" @default.
- W2351776761 hasPublicationYear "2010" @default.
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