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- W2351797030 abstract "To know the dominant pathogens of potato Fusarium dry pot in Gansu,tubers showing symptoms of dry rot were sampled at farmers' storage caves in Zhangye city,Tianzhu,Yongdeng,Lintao,Weiyuan and Xihe county from Feb.2006 to Mar.2007.The pathogens were isolated by tissue isolation method,the Fusa-rium obtained were identified according to the taxonomic system of Nelson after being sub-cultured and single-spored.The results showed that the total 293 Fusarium isolates were obtained from the 12 storage caves in six sampling sites,and the dominant species were F.sambucinum and F.solani.The results also indicated that the dominant species were different in different ecological areas,for example,F.solani was the dominant pathogen in Zhangye,Tianzhu and Weiyuan,and where its isolation frequencies were 42.6%,42.1% and 32.4% respectively,and that of F.sambucinum were 14.8%、5.3% and 26.5% respectively.F.sambucinum was the dominant pathogen in Yongdeng,Lintao and Xihe,and where its isolation frequencies were 52.1%、50.9% and 55.2%,and that of F.solani were 23.3%、32.7% and 20.7%.The morphological characteristics of these two species on PDA and CLA were observed and described in details in this paper.According to Koch's Postulate,the pathogenicity of the dominant pathogen were tested with mixed isolates on potato varieties Atlantic,Shepody and a local variety from Tongwei county.It was verified that both of the two dominant Fusarium species were pathogenic to potato tubers.Genomic DNA was isolated from pure cultures of GAUF-F12 strain of F.sambucinum and amplified with primer pairs EF-1α(EF-1H and EF-2T).The PCR product was then sequenced,and aligned in GenBank.It was indicated that there was a very close relationship between GAUF-F12 and the 5 F.sambucinum isolates,and the max similarity of their sequences was 99%.DNASTAR was used to draw the phylogenetic tree of isolate GAUF-F12.It was showed that strain GAUF-F12 and the above 5 F.sambucinum isolates were in the same cluster,which was consistent with the results of morphological identification." @default.
- W2351797030 created "2016-06-24" @default.
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- W2351797030 date "2011-01-01" @default.
- W2351797030 modified "2023-09-28" @default.
- W2351797030 title "Isolation and identification of the dominant pathogens causing potato Fusarium dry rot in Gansu Province" @default.
- W2351797030 hasPublicationYear "2011" @default.
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