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- W2351799319 abstract "Adiponectin(Adp) is an adipocytokine maily secreted by adipocytes. Evidence suggests adiponectin action on the reproductive system. Recent researches in roden showed that Adp inhibits LH secretion mediated by AdpR in pituitary. The aims of this study were to observe the correlation and developmental patterns of adiponectin receptors and reproduction-related factors mRNA expressions in pituitary of Wannahua pigs. The test animals were sampled on 0, 30, 45, 90, and 180 d of age, including 5 boars and 5 sows at each different days of age The development patterns of adiponectin receptors(AdpR1, AdpR2), Gonadotropin Releasing Hormone Receptor(GnRHR), Luteinizing Hormone(LH-β), and Follicle Stimulating Hormone(FSH-β) mRNA in pituitary were investigated and their correlation were analyzed by Real-time fluorescent quantitative PCR(RT-PCR) while the concentration of LH and FSH in serum at different days of age were detected by ELISA. The results showed that: 1) The expression of AdpR2 mRNA were significantly changed with age increasing(P0.05); The mRNA expression of AdpR1 in different days were significantly higher than that of AdpR2 at any days of age, while the expression of AdpR1 at birth、30 and 180 d of boars were significantly higher than that of those of sows. 2) The expression of LH-β at different growth stages and FSH-β mRNA in different days of sows were significantly changed with age increasing(P0.05); The expression of FSH-β at birth of sows was significantly higher than that of boars(P0.05) and GnRHR at 90 d of boars was significantly higher than that of sows(P0.05). The LH and FSH level in serum were significantly changed with age increasing(P0.01). The FSH contents in different days of sows were higher than those of boars. 3)The expression of AdpR1 in different days of boars were negatively correlated with GnRHR, FSH-β and LH-β respectively(P0.05). The expression of AdpR2 of boars were negatively correlated with GnRHR(P0.05). The expression of AdpR2 in different days of sows were negatively correlated with GnRHR、LH-β mRNA and FSH level in serum respectively(P0.01 or P0.05). Furthermore, the mRNA expression of AdpR1 and AdpR2 were significant positive correlation(P0.01 or P0.05)both in boars and in sows and GnRHR were significantly positive correlated with FSH-β(P0.05)and LH-β(P0.01) respectively. Our results indicated that the adiponectin may be mediated by AdpR and inhibit secretion of FSH and LH by GnRHR or directly in pituitary." @default.
- W2351799319 created "2016-06-24" @default.
- W2351799319 creator A5048944139 @default.
- W2351799319 date "2013-01-01" @default.
- W2351799319 modified "2023-09-27" @default.
- W2351799319 title "The Expression of Adiponectin Receptors(AdpR1 and AdpR2), Gonadotropin Releasing Hormone Receptor(GnRHR), Follicle Stimulating Hormone(FSH-β) and Luteinizing Hormone(LH-β) mRNA in the Pituitary of Wannanhua Pigs" @default.
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