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- W2351864633 abstract "AIM: To construct and express the fused vector of ldh gene of Streptococcus Mutans and the encoding gene ctxB of cholera toxin B subunit.METHODS: The gene ldh and ctxB were amplified by PCR,and integrated into the vector pET32a(+).The recombinant plasmids were identified by restriction endonuclease digestion,PCR and sequencing.Plasmids were transformed into E.coli BL21(DE3)and induced by IPTG.RESULTS: The 1.4 kb fused gene ldh-ctxB was obtained by restriction endonuclease digestion and PCR.The homoeology was 98% between sequence ldh in the vector pET-LDH/CTB and ldh in GeneBank,and ctxB was 99% by sequence analysis and acomparison.The recombinant plasmid expressed the fusion protein of 70 KD.CONCLUSION: The fused vector of ldh and ctxB was constructed and expressed correctly." @default.
- W2351864633 created "2016-06-24" @default.
- W2351864633 creator A5053571254 @default.
- W2351864633 date "2012-01-01" @default.
- W2351864633 modified "2023-09-23" @default.
- W2351864633 title "Construction and expression of the fused Vector of Lactate dehydrogenase of Streptococcus Mutans and cholera toxin B subunit" @default.
- W2351864633 hasPublicationYear "2012" @default.
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