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- W2351882065 abstract "OBJECTIVE To evaluate the method of inducing the directional differentiation of embryonic stem(ES) cells into cardiomyocytes using icariin(ICA) in vitro.METHODS With Hanging-drop cultures of ES cells,ICA was used as an inducer of the directional differentiation of ES cells into cardiomyocytes.The expressions of cardiac-specific genes and sarcomeric proteins were verified by semi-quantitative RT-PCR and immunocytochemistry analysis.RESULTS ICA at 10-7mol·L-1 significantly enhanced cardiac differentiation,and up to 84% of the beating embryoid bodies(EBs) was found.The expressions of α-cardaic MHC and MLC-2v genes were detected in cardiomyocytes derived from EBs on early differentiation stage.Furthermore,the differentiated beating cardiac cells were stained positively with anti-α-actinin mAb and anti-troponin T mAb.CONCLUSION An effective method was established by combining drug with hanging-drop cultures of ES cells for inducing the differentiation of ES cells into cardiomyocytes in vitro." @default.
- W2351882065 created "2016-06-24" @default.
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- W2351882065 date "2007-01-01" @default.
- W2351882065 modified "2023-09-28" @default.
- W2351882065 title "Study on Induction of Icariin on Directional Differentiation of Embryonic Stem Cells into Cardiomyocytes in Vitro" @default.
- W2351882065 hasPublicationYear "2007" @default.
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