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- W2351892007 abstract "Objective To study the regulation of microRNA on Prestin gene,a rat Prestin-3'UTR dual-luciferase reporter gene vector was constructed. Methods The 3'untranslated region( 3'UTR) fragment of SLC26A5 gene was amplified by PCR from genomic DNA of C6 cells. PCR products were cloned into pmir-RB-REPORTTMdual-luciferase reporter gene vector. DNA sequencing identified 3'UTR of SLC26A5 and the dual-luciferase reporter gene vector was transferred into 293 T cells to quantitate the reporter activity by the Dual-Luciferase Reporter Assay System. Results The results showed that a 3' UTR fragment of SLC26A5 gene was successfully cloned into the pmir-RB-REPORTTMdual-luciferase reporter gene vector,which authenticated by DNA sequencing,and the luciferase activity of 293 T cells which was transfected vector could expressed availably. Conclusion Rat Prestin-3'UTR dual-luciferase reporter gene vector was successfully constructed." @default.
- W2351892007 created "2016-06-24" @default.
- W2351892007 creator A5067358577 @default.
- W2351892007 date "2015-01-01" @default.
- W2351892007 modified "2023-09-26" @default.
- W2351892007 title "Constructing and identifying of rat Prestin-3'UTR dual-luciferase reporter gene vector" @default.
- W2351892007 hasPublicationYear "2015" @default.
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