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- W2351892008 abstract "In order to study the purification and renaturation of rPA(K) inclusion body in the prokaryotic system,the dialysis renaturation and dilution renaturation was used to renature the target product respectively.Through dialysis renaturation,the specific activity was 1.33×105 IU/mg;while it was 1.06×105 IU/mg by using dilution renaturation.The renatured rPA(K) was further purified by S·TagTM rEK purification kit,and the two kinds of eluent(rEK and MgCl2) were compared.SDS-PAGE indicated that the molecular weight of target protein had no change by using MgCl2 and was still 43 ku.While,by using rEK,the molecular weight of it was 40 ku.That meant the non-target fragment was cut off,and the purity of product reached 72 %." @default.
- W2351892008 created "2016-06-24" @default.
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- W2351892008 date "2009-01-01" @default.
- W2351892008 modified "2023-09-22" @default.
- W2351892008 title "The Purification and Renaturation of rPA(K) Prokaryotic Expressive Product" @default.
- W2351892008 hasPublicationYear "2009" @default.
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