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- W2352016298 abstract "Suppression subtractive hybridization(SSH) is a powerful means to identify the differentially expressed genes including low,rare abundance transcripts.In this study,SSH was used to identify the cold tolerance-related genes in the brain of common carp(Cyprinus carpio).The materials were prepared by cooling the cross F_(2) of purse red carp(Cyprinus carpio wuyanensis Tchang) cold-tolerant strains and Boshi carp(Cyprinus pellegnini Tchang) to different temperatures.After isolation of total RNA,double strand cDNAs were synthesized and digested with restriction enzyme Rsa Ⅰ.The digested tester was then ligated to two different adaptors.Following the subtractive hybridization between the tester and the driver,a subtracted cDNA library,where enriched genes were expressed differentially under the lower temperature,was then constructed containing 2000 clones.Among 480 clones detected by dot blotting,60 positive clones were determined to differentially express,and 29 clones were sequenced and aligned on Internet,which showed higher identities among 13 clones.The major differentially expressed genes included brevican genes,AMP-acid ligase,muscle cofilin 2(CFL 2) gene,isotocin gene,MHC class Ⅱ β chain,zinc finger protein,cytochrome oxydase,EPD-1 gene,sup-related nuclear receptor/retinoid signaling modulator and so on.Nine genes were induced up regulated,indicating the variation of regulating genes to adapt the lower temperature.Four genes were down regulated,indicating the reduced metabolic activities during the cooling process.This study plays an important role in researching the molecular genetic mechanism of fish cold tolerance at the molecular level." @default.
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- W2352016298 date "2006-01-01" @default.
- W2352016298 modified "2023-09-23" @default.
- W2352016298 title "Application of suppression subtractive hybridization(SSH) to study of cold tolerance in common carp(Cyprinus carpio)" @default.
- W2352016298 hasPublicationYear "2006" @default.
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